Process

  • Take a diploid cell line (probably ESC or iPSC or PGCLC)
  • Induce meiosis and form many haploid cell lines
  • Genotype the haploid lines and select the best ones.
  • Fuse two haploid cells to re-generate a diploid cell line.
  • Repeat as desired. At the end, either differentiate the cells into oocytes or perform nuclear transfer into a donor oocyte.

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